83-5972-46　IGF-I Receptor beta (Insulin-Like Growth Factor I Receptor beta, IGF1Rb) 100ul　592429

特徴

• Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3).
• Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands.
• Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4).
• Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6).
• Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop.
• Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7).
• Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).
• Applications:Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000.
• Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight
• Immunoprecipitation: 1:200Immunofluorescence (IC): 1:200-1:800Flow Cytometry (fixed/permeabilized): 1:200-1:800Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.