83-5972-29　S6 Ribosomal Protein, phosphorylated(Ser240/244) 100ul　592412

特徴

• To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation.
• Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein.
• Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions.
• This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors.
• The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a 19 amino acid region in the S6 carboxy-terminus.
• Applications:Suitable for use in Immunofluoresence/Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.
• Immunofluorescence (IC): 1:800Flow Cytometry (Fixed/Permeabilized): 1:400-1:1600Immunohistochemistry (paraffin): 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.