To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2).
Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein.
Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2).
This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3).
The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Fixed/Permeabilized): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 2mg/mL BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes endogenous levels of human ribosomal protein S6 only when phosphorylated at Ser235 and 236. Species Crossreactivity: mouse, rat, monkey, monkey and S. cerevisiae
Clone # USB：8A30(D57.2.2E)
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu Mk Mo Rt Ye
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Species sequence homology: chicken and porcine (100%)
