83-5971-39　Volociximab Biosimilar, Human ITGA5 (CD49e) 100ug　592308

特徴

• Volociximab is a high-affinity IgG4 chimeric (82% human, 18% murine) monoclonal antibody that specifically binds to α5β1 integrin.
• Integrins are a superfamily of widely expressed transmembrane glycoprotein receptors for extracellular matrix ligands, such as fibronectin, vitronectin, laminin, collagens, and other plasma membrane proteins, and function in the regulation of a broad variety of cellular processes, including embryogenesis, inflammation, bone metabolism, apoptosis, cell proliferation, angiogenesis, and tumor metastasis.
• Relevant preclinical models for the mechanism of action and antitumor activity evaluation were selected based on the cross-reactivity of volociximab to the non-human α5β1 homologues.
• Volociximab and its parent mouse antibody, IIA1, do not cross-react with murine α5β1 integrin, but do cross-react and block the chicken and cynomolgus monkey target protein.
• Volociximab inhibited human umbilical vein endothelial cells from forming tube-like vessel structures in a three-dimensional fibrin matrix in vitro and was independent of the growth factor stimulus.
• These data suggest that the α5β1 signaling pathways are downstream of growth factor stimulation.
• Moreover, volociximab inhibited growth factor–stimulated human neonatal foreskin vascular growth when grafted into severe combined immunodeficient mice in vivo.
• In addition, volociximab inhibited vessel formation and human tumor xenograft growth in the chicken chorioallantoic membrane model in ovo.
• Finally, in a preclinical model of choroidal neovascularization in cynomolgus monkeys, volociximab was a potent inhibitor of angiogenesis.
• Applications:Suitable for use in ELISA, Neutralization, Functional Assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by Volociximab.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.