Responsible for binding to the receptor(s) on target cells.
Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into dendritic cells (DCs) and endothelial cells .
Binding to the macrophage specific lectin CLEC10A also seem to enhance virus infectivity.
Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, although results are contradictory.
Members of the Tyro3 receptor tyrosine kinase family also seem to be cell entry factors in filovirus infection.
Once attached, the virions are internalized through clathrin-dependent endocytosis and/or macropinocytosis.
After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, allowing its binding to the host entry receptor NPC1 and unmasking its fusion peptide to initiate membranes fusion.
Applications:Suitable for use in ELISA and SDS-PAGE.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：human
Grade：Highly Purified
Purity：≥95%
Form：Supplied as a liquid in PBS, pH 7.5.
Specificity：Recognizes Ansuvimab.
Isotype：IgG1
Calc Applications Abbrev：E
Immunogen：Recombinant protein corresponding to humanized GP1 surface protein of Ebolavirus, expressed in CHO cells.
