S100 protein is a 21,000-Da component first isolated from brain by Moore (1965).
Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988).
The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b.
S100 genes are clustered on human chromosome 1q21.
S-100 protein expression by primary and metastatic adenocarcinomas.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Grade:Ascites
Purity:Ascites
Form:Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1mL sterile PBS.
Specificity:Recognizes S100 beta Subunit. Species Crossreactivity: human, rabbit and rat.
Clone # USB:SH-B1
Isotype:IgG1
Calc Applications Abbrev:IHC
Calc Crossreactivity:Hu Rb Rt
Immunogen:Bovine brain S-100b. Species sequence homology: equine and monkey