83-5962-85　Uroplakin III (Uroplakin-3a, UP3a, Uroplakin III, UPIII, UPK3A, UPK3) 1mL　590555

特徴

• 590555 reacts specifically with uroplakin IIIA present in the superficial cell layer of the urothelium.
• The binding region has been localized on the extracellular part of the antigen.
• Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells.
• Uroplakin is a membrane glycoprotein (47kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al.
• 1993 & 1194).
• About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III.
• Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al.
• 1995).
• Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (paraffin): 1:20-1:60 (1.6-5ug/ml; microwave treatment recommended).
• Strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra
• Western Blot: 1:4000-1:20,000 (0.005-0.025ug/ml)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.