Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system.
The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma.
WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor.
Lung adenocarcinomas rarely stain positive with this antibody.
WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RT, Requires HIER
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1mg/mL BSA, 0.05% sodium azide.
Specificity：Recognizes human WT1. Species Crossreactivity: rat
Clone # USB：WT1/1434R (recomb)
Isotype：IgG,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human WT1. Localization: Nuclear, cytoplasmic
