von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets.
It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation.
vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds.
378674 helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g.
Kaposi's sarcoma and cardiac myxoma.
It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug lysateImmunofluorescence: 1-2ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RTOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1mg/mL BSA, 0.05% sodium azide.
Specificity：Recognizes human von Willebrand Factor.
Clone # USB：rVWF/1465
Isotype：lgG1,k
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1815-1939 from human von Willebrand factor.
