Mitogenic platelet derived growth factor (PDGF) signaling is mediated by the PDGF tyrosine kinase receptors PDGFRα and PDGFRbeta, which exist as homo- or heterodimer isoforms (alpha alpha, beta beta, alphabeta).
Secretion of PDGFs and expression of PDGFRs in a broad distribution of cell types contributes to regulation of cellular processes including cell growth, actin reorganization, prevention of apoptosis, and chemotaxis.
Structurally, the transmembrane PDGFRs are composed of an N-terminal extracellular domain with five immunoglobulin repeats, and an intracellular domain containing two spaced tyrosine kinase domains.
PDGF ligands display preferential affinity to PDGFRalpha and PDGFRbeta with PDGFRbeta activation restricted to binding by PDGF BB and PDGF DD.
Growth factor binding initiates receptor dimerization and activates the cytosolic tyrosine kinase domains.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and electron microscopy.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml, ECLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PDGF receptor beta. Species Crossreactivity: mouse and rat
Clone # USB：42G12
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresonding to human PDGF Receptor beta.
