Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine and milk, it is also found in malignant and atherogenic tissues.
Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival.
Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon and lung cancers as well as metastatic carcinomas.
The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis.
In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack.
Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: frozen and paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Osteopontin.
Clone # USB：53
Isotype：IgG2a
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Osteopontin.
