Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression.
With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope.
The Myc-Tag (EQKLISEEDL) corresponds to the partial peptide sequence of the human c-myc gene product.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml, ECLImmunoprecipitation: 5ug/mlImmunocytochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Specificity：Recognizes the Myc-tag of Myc-tagged recombinant proteins.
