Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin sections): fixation in alcohol-based fixatives, such as methacarn, is recommended.
Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained.
For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37℃ for 2 to 4 hours.
Optimal conditions must be determined individually for each application.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：6mL
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from hybridoma tissue culture supernatant.
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes actin isotypes alpha and gamma of skeletal, cardiac and smooth muscle. This antibody labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas but does not react with non-myogenic sarcomas. It is nonreactive with other neoplasms such as carcinoma or lymphoma and with other mesenchymal and all epithelial cells, with the exception of myoepithelium. Species Crossreactivity: monkey and rabbit
Clone # USB：HHF35
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk Rb
Immunogen：Human myocardium (proteins extracted in 2% SDS).
