83-5962-35　gp100 100ug　590374

特徴

• Melanocytes produce organelles called melanosomes which produce melanin, the pigment that gives color to skin, hair, eyes, scales and feathers.
• gp100 was identified in an attempt to clone the gene Tyrosinase, an enzyme required for melanin synthesis.
• Further testing determined that gp100 is a melanoma-specific protein and is responsible for melanosome maturation, facilitating the transition from amorphous rounded vesicles to fibrillary ellipsoid organelles.
• Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone.
• It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms.
• 590374 stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma.
• It also stains angiomyolipomas, tumors most commonly associated with the kidney.
• Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative.
• This gp100 antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• The gp100 molecule is a 100kD glycosylated protein that is cleaved into a small (26kD) carboxy-terminal fragment and a larger amino- terminal section (60-64kD), which is subsequently cleaved to generate 26kD and 34-38kD fragments.
• Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT.
• Requires HIERWestern Blot: 1-2ug/mlFlow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 0.5-1ug/mlOptimal dilution determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.