The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4.
Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e.
the D1-D2 domains of the protein, 36kD) and LAG-3V3 (D1-D3, 52kD).
The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome.
LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry.
Recommended Dilution:Western Blot: 5ug/mlImmunoprecipitation: 10ug/mlImmunohistochemistry (FFPE): 1:150Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified from tissue culture supernatant.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Specificity：Recognizes the 30aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Clone # USB：L4-PL33
Isotype：IgG1,k
Calc Applications Abbrev：E FC IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 30aa, GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY from the first N-terminal D1 domain of human LAG-3.
