The Stress-activated protein kinases alpha and gamma (SAPKalpha and gamma), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinases belonging to the MAP kinase family.
The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others.
Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.05ug/mlWestern Blot (ECL): 0.5ug/mlImmunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Thiophilic adsorption and size exclusion chromatography
Form：Supplied as a lyophilized powder in PBS, 0.09% sodium azide, PEG and sucrose. Reconstitute with 1mL sterile dH2O.
Specificity：Recognizes human Jnk2. Species Crossreactivity: mouse and canine
Clone # USB：12C5
Isotype：IgG1
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide corresponding to the sequence near the N-terminus of human Jnk2.
