The reagent is intended as an isotype control to establish the amount of non-specific antibody binding.
For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody.
Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using.
If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Applications:Suitable for use as an isotype control in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ before opening.
DO NOT FREEZE! Stable at 4℃ as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months at 4℃.
Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
PE conjugates are sensitive to light.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：590305 with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
