83-5961-86　EGF Receptor 100ug　590163

特徴

• The epidermal growth factor receptor (EGFR) is a 170kD membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region and a tyrosine kinase domain-containing cytoplasmic region.
• Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules.
• Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g.
• SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation.
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 1:100 (final concentration)Western Blot: 1:1000-1:2000Immunocytochemistry:1:100Immunoprecipitation: 2-5ul/250ul of cell extract from 2.5 x 106 cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.