Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: fixation in alcohol-based fixatives, such as methacarn, is recommended.
Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained.
For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37℃ for 2 to 4 hours.
Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes "high molecular weight" cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50kD, respectively, which are characteristically found in "complex" epithelium. The antibody reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. This antibody reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma.
Clone # USB：34bE12
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Keratin solubilized from human stratum corneum.
