Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended.
Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained.
Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection).
A 20- to 50-fold dilution of the antibody is suggested.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes “high molecular weight” cytokeratin 1, 68kD, of “complex” epithelium. The antibody reacts with all squamous epithelium and squamous carcinomas. It does not react with adenocarcinomas of either simple epithelium (hepatoma) or ductal origin (breast, pancreas).
Clone # USB：34bB4
Isotype：IgG1
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Keratin solubilized from human stratum corneum.
