Cyclins are a family of proteins which function as the regulatory subunits of cyclin/cyclin-dependent kinase (Cdk) holoenzymes, mediating entry into and progression through the cell cycle.
Each cyclin is expressed in a temporal fashion during the cell cycle, partnering with a specific Cdk to activate its protein kinase activity.
The most well defined mitotic cyclins are: cyclin D, which partners with cdks 4 and 6 during early G1 phase; cyclin E, which activates Cdk2 in the G1 to S phase transition; cyclin A, which utilizes Cdks 1 and 2 during progression through S phase and into G2; and cyclin B, which activates Cdk1 in M-phase promotion.
Applications:Suitable for use in Western Blot, Immunoprecipitation, immunohistochemistry, Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml, ECLImmunohistochemistry (frozen/paraffin): 10ug/ml Immunoprecipitation: 1-10ug/200ug cell extract from 5x106 cellsFlow Cytometry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Specificity：Recognizes human Cyclin D1. Species Crossrectivity: mouse
Clone # USB：5D4
Isotype：IgG2a
Calc Applications Abbrev：FC IC IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human cyclin D1.
