In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14-Thr and 15-Tyr.
This maintains the inactive state until the end of G2-phase.
The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14-Thr and 15-Tyr of p34cdc2 rendering the complex active at the G2/Mboundary.
This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded.
This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis.
Therefore cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：hamster
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Reognizes hamster Cyclin B1. Species Crossrectivity: human, mouse, ratcanine, guinea pig, hamster, porcine, rabbit and sheep
Clone # USB：V63
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Gp Hm Hu Mo Po Rb Rt Sh
Immunogen：Synthetic peptide corresponding to hamster cyclin B1 at C-terminal.
