The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins.
These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates.
Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia.
Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1-5ug/ml, ECLImmunoprecipitation: 3ug/200-300ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Specificity：Recognizes human Cdc25A. Species Crossreactivity: mouse and rat
Clone # USB：DCS-120
Isotype：IgG2a
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Cdc25A.
