CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells.
Multiple isoforms of CD45R are distributed throughout the immune system according to cell type.
These isoforms arise because of alternative splicing of exons 4, 5, and 6.
The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out).
The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.
CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
This antigen is also expressed on the surface of cells of myeloid lineage.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlFlow Cytometry: 0.5-1ug/106 cells in 0.1 mlImmunofluorescence: 0.5-1ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 0.5-1ug/ml for 30 min at RTOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/mL BSA, 0.05% sodium azide.
