70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43.
No reactivity has been observed with reactive B-cells.
So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population.
When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained.
Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 0.5-1ug/106 cells in 0.1 mlImmunofluorescence: 1-2ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 0.5-1ug/ml for 30 min at RTOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/mL BSA, 0.05% sodium azide.
Specificity：Recognizes human CD43.
Clone # USB：SPN/1766R
Isotype：IgG,k
Calc Applications Abbrev：FC IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human CD43.
