CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues.
It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.
Applications:Suitable for use in Immunohistochemistry and Flow Cytometry.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Flow Cytometry: 1-2ug/10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, 0.1mg/mL BSA, 0.05% sodium azide.
Specificity:Recognizes human CD20. Does not react with canine.
Clone # USB:IGEL/1497R
Isotype:IgG
Calc Applications Abbrev:FC IHC
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human full-length CD20.