83-5961-51　Beta-Catenin 100ug　589971

特徴

• Beta-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt signaling, glycogen synthase kinase-3beta (GSK-3beta) phosphorylates any free β-catenin at the amino terminus.  This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated beta-catenin degradation via the ubiquitin-proteosome pathway.  Wnt signaling stabilizes beta-catenin by inhibiting the activity of GSK-3beta and preventing beta-catenin degradation.  Beta-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote  expression of a variety of target genes, including c-myc and cyclin D1.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml, ECLImmunohistochemistry: 1-5ug/ml Immunoprecipitation: 2ug/200-300ul cell extractOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.