The Bcl-2 family of proteins regulate the intrinsic apoptotic pathway and are characterized by the sharing of at least one of four conserved Bcl-2 Homology (BH) domains.
Bcl-2 family members function as either pro- or anti-apoptotic factors, and are generally categorized into three groups based on structural homology and function.
Bcl-2-like proteins (Bcl-2, Bcl-xL, Bcl-w, A1, and Mcl-1) are pro-survival.
Pro-apoptotic members can be sub-divided into two groups: the multi-BH domain group, including Bax, Bak, and Bok; and the more numerous and diverse BH3-only group, which includes Bim, Bad, and Bid.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1ug/ml, detects a band of ~26kDFlow Cytometry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Specificity：Recognizes rat Bcl-2. Species Crossreactivity: human, mouse and drosophila.
Clone # USB：83-8B
Isotype：IgG1
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Dr Hu Mo Rt
Immunogen：Recombinant protein corresponding to rat Bcl-2 beta.
