Bassoon is a 420kD protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle.
Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.
These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 - 1:1000Immunoprecipitation: 2.5ugImmunocytochemistry: 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat Bassoon. Species Crossreactivity: human, mouse, rat, bovine and canine
Clone # USB：SAP7F407
Isotype：IgG2a,k
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Bo Ca Hm Mo Rt
Immunogen：Recombinant protein corresponding to rat Bassoon.
