Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system.
Human ApoE is an apolipoprotein that has three isoforms: ApoE2, ApoE3, and ApoE4.
The ApoE4 variant is the largest known genetic risk factor for late-onset Alzheimer's disease, and while the exact mechanism remains to be determined, it appears there may be an interaction with beta-amyloid.
Alzheimer's disease is characterized by build-ups of aggregates of the peptide beta-amyloid.
Apolipoprotein E enhances proteolytic break-down of this peptide, both within and between cells.
The isoform ApoE4 is not as effective as the others at promoting these reactions, resulting in increased vulnerability to AD in individuals with that gene variation.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human ApoE4 protein. Does not cross-react with ApoE2 or ApoE3.
Clone # USB：4E4
Isotype：IgG1,k
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to Arg110 of human ApoE4 protein.
