anthracis is composed of three proteins: a cell-receptor binding protein known as protective antigen (PA), and two enzymes, Edema Factor (EF) and Lethal Factor (LF).
An 83kD form of PA binds to a cell surface receptor and, upon proteolytic activation to a 63kD fragment, heptamerizes to form a membrane channel that mediates the entry of three molecules of LF or EF into the cells.
PA and LF form Lethal toxin, which is the dominant virulence factor produced by B.
Anthracis and is the major cause of death in infected animals.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:2000Immunoprecipitation: 1:10-1:500Western Blot: 1:100-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, 0.01% sodium azide.