ACTH (adrenocorticotropic hormone, corticotropin) is a 39aa active peptide produced by the anterior pituitary.
POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267aa polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases.
POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (melanocyte stimulating hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), lipotropin-beta, lipotropin-gamma, beta-endorphin and Met-enkephalin.
ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.
Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease.
It reacts with ACTH-producing cells (corticotrophs).
It also may react with other tumors (e.g.
some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (formalin-fixed paraffin embedded): 0.5-1ug/ml for 30 min at RT.
Requires HIER
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1mg/mL BSA, 0.05% sodium azide.
Specificity：Recognizes human Synacthen aa1-24 of ACTH; does not cross-react with CLIP aa17-39 of ACTH. Species Crossreactivity: mouse and rat
Clone # USB：r57 (recomb)
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：N-terminal peptide within aa1-24 from the Synacthen/ACTH region of human POMC.
