KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A.
KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain.
The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively.
A 189 aa isoform diverges at aa 186.
KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression.
It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 3-25ug/ml using immersion fixed paraffin-embedded sections of human ronsilFlow Cytometry: 0.25ug/10e6 cells using human peripheral bloodOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from cell culture supernatant.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human KLRG1.
Clone # USB：2388C
Isotype：IgG
Calc Applications Abbrev：FC IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu60-Phe195 from human KLRG1, expressed in CHO cells.
