83-5961-35　ATPase alpha 3, Na, K (Azide & BSA-Free) 3mg　A4000-65C1

特徴

• The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane.
• This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA).
• The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit.
• Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
• The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression.
• The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.
• The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.
• Applications:Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Blocking/Neutralization.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 5ug/mlWestern Blot: 1ug/ml detects a band at~110kD from canine skeletal muscle extract
• Immunohistochemistry (Frozen): 3ug/ml, Staining of rat retina yeilds a pattern consistant with plasma membrane localization
• Immunohistochemistry (Paraffin): 1:10-1:500Flow Cytometry: 1ug /10e6cellsOptimal dilutions to be determined by the researcher.
• Positive Control:Human tonsil and prostate, rat retina
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.