83-5960-59　In vivo Grade Recombinant MOPC-21 Canine IgG3 Isotype Control 1mg　587248

特徴

• The MOPC-21 antibody (IgG1, kappa) has been used for a long time as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody.
• The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein.
• The recombinant MOPC-21 IgG1 kappa isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study.
• Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study.
• The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa.
• Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery.
• Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies.
• Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.
• As an ideal recombinant mouse IgG1 isotype control, the recombinant MOPC-21 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions.
• The mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.
• The in vivo grade recombinant MOPC-21 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
• Recombinant isotype control antibodies and Fc silenced mutants from clone MOPC-21 good for in vitro and in vivo studies.
• The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein.
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and in vivo animal model research.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: frozen/paraffinOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.