83-5959-97　In vivo Grade Recombinant B1-8 Ferret IgG1 Isotype Control 1mg　587162

特徴

• The anti-NP B1-8 antibody (IgG2c, kappa) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody.
• The recombinant B1-8 IgG2c kappa isotype control and mutants (natural and engineered mIgG2c isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study.
• The mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does express the mouse IgG2a antibody, and vice versa.
• If one uses any of the mouse strains for animal research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies.
• There are more and more IgG antibody formats with Fc engineered.
• The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular.
• Thus, the wild type mouse IgG2c isotype control and the Fc silenced mouse IgG2c isotype control mutants should be used in such an application.
• The in vivo grade recombinant B1-8 mouse IgG2c isotype control antibody (mIgG2c isotype control) was produced in mammalian cells.
• Recombinant anti-NP isotype control antibodies and Fc silenced mutants from clone B1-8 good for in vitro and in vivo studies.
• Low or no specific binding to human, rat, and mouse samples tested.
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and in vivo animal model research.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: frozen/paraffinOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.