Collagen alpha-1(VII) chain (UniProt: Q02388; also known as Long-chain collagen, LC collagen) is encoded by the COL7A1 gene (Gene ID: 1294) in human.
LH24 targets the basement membrane zone of keratinocytes, specifically Type VII Collagen non-collagenous domain 2.
Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts.
However, epithelial cells may also synthesize small amounts of collagen.
The collagen super family includes over 20 different types of collagen with at least 38 distinct polypeptide chains.
Collagen type VII is primarily synthesized by keratinocytes and skin fibroblasts.
It is composed of three identical alpha chains and its distribution is restricted to stratified squamous epithelial basement membrane.
It contributes to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins.
Type VII collagen contains a non-collagenous domain 1 (NC1), a central collagenous domain, and a non-collagenous domain 2 (NC2) that catalyze the anchoring fibril assembly.
Mutations in COL7A1 gene have been linked to epidermolysis bullosa dystrophica, a blistering skin disease characterized by tissue separation that occurs below the dermal-epidermal basement membrane at the level of the anchoring fibrils.
(Ref.: Siprashvili, Z., et al.
(2016).
J.
Am.
Med.
Assoc.
316(17); 1808-1817; Chung, HJ., and Uitto, J (2010).
Dermatol.
Clin.
28(1); 93-105).
Applications: Suitable for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:25 detected Collagen, Type VII in frozen human skin tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Collagen alpha-1(VII) chain. It targets an epitope within the C-terminal noncollagenous domain (NC2). Species Crossreactivity: human
Clone # USB：LH24
Isotype：IgM,k
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Crude extract from human skin. Epitope: C-terminal
