SIGLEC8 is a sialic acid binding lectin similar to CD33.
In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein.
SIGLEC8 is expressed e.g.
in lymph nodes and spleen.
It is an eosinophil marker, although it can be found also on the surface of mast cells.
Crosslinking of SIGLEC8 leads to apoptosis.
Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
Applications: Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human SIGLEC8.
Clone # USB：7C9
Isotype：IgG1
Calc Applications Abbrev：E FC IP
Calc Crossreactivity：Hu
Immunogen：Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
