The 5-Hydroxymethylcytosine antibody, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine.
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base.
It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group.
Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type, generally, the levels of 5-hydroxymethylcytosine increase with age.
Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Dot Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
Specificity：Recognizes 5-hydroxymethyl cytosine (hmc, 5hmc). Does not react with 5-methylcytosine or unmethylated cytosine.
Clone # USB：AB3/63.3
Isotype：IgG2a,k
Calc Applications Abbrev：DB IF IP
Immunogen：5-Hydroxymethylcytosine conjugated to BSA.
