G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell.
It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma.
G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.2-0.4ug/ml.
When used in conjunction with compatible secondary reagents the detection limit for recombinant human G-CSF is 1.5-2ng/lane under non-reducing conditions, and 7.5-10ng/lane under reducing conditions
ELISA (100ul/well): 2-4ug/ml will detect at least 100pg/ml of recombinant human G-CSF when used with G8950-26A as the detection antibody at 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from cell culture.
Form：Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes human G-CSF.
Clone # USB：16k127
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human G-CSF, expressed in E. coli.
