Human troponin I is presented in cardiac muscle tissue by a single isoform with molecular weight 23876Da and it consists of 209aa residues.
cTnI molecule contains two serines in the 22 and 23 positions.
Both amino acid residues can be phosphorylated in vivo by protein kinase A, so four forms of protein - one dephospho, two monophospho and one bisphospho - can coexist in the cell.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.