MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion.
Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion.
Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential.
Applications:Suitable for use in Western Blot, Electron Microscopy, Immunoprecipitation, Immunofluorescence and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Hybridoma:Spleen cells from immunized BALB/c mice were fused with cells of the NS1 myeloma cell line.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Specificity：Recognizes canine CD107b, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein. Demonstrates staining pattern consistant with localization to lysosome. Species Crossreactivity: human and mink. Does not react with mouse or rat
