Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Recommended Negative Controls: Mouse IgG1 Negative Control
This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells.
Detects an antigen of 90kD.
Clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P.
falciparum to ICAM-1.
Removal of sodium azide is recommended prior to use in functional assays.
Applications:Flow Cytometry: Neat-1:10Immunohistochemistry: (frozen)- 1:20-1:50Immunoprecipitation: 5ug / 10e7 cellsOptimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the D1 domain of ICAM-1. It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes.
