83-5958-79　Pregnancy Associated Plasma Protein A (PAPP-A, Pappalysin 1, PAPA, PAPPA, PAPP-A, PAPPA1, Aspecific BCL2 ARE-binding Protein 2, ASBABP2, Differentially Placenta 1 Expressed Protein, DIPLA1, EC 3.4.24.79, IGF-dependent IGFBP-4 Protease, IGFBP-4ase, Insulin-like Growth Factor-dependent IGF-binding Protein 4 Protease, Pappalysin 1 Precursor) (HRP) 100ul　P6000-82-HRP

特徴

• PAPP A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500kD, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP).
• In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity.
• PAPP A is widely used as a marker of some pathologies during pregnancy.
• Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (Trisomy 21).
• Applications:Suitable for use in the detection of human PAPP-A antigen in ELISA and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Hybridoma:Hybridization of SP 2/0 myeloma cells with spleen cells from Balb/c mice.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.