PAPP A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500kD, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP).
In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity.
PAPP A is widely used as a marker of some pathologies during pregnancy.
Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (Trisomy 21).
Applications:Suitable for use in the detection of human PAPP-A antigen in FLISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Hybridoma:Hybridization of SP 2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:May be stored at 4℃ before opening.
DO NOT FREEZE! Stable at 4℃ as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Allophycocyanin (APC) is light sensitive.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affintiy chromotography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits).
Clone # USB：1.B.132
Isotype：IgG2b
Calc Applications Abbrev：E FLISA WB
Calc Crossreactivity：Hu
Immunogen：Purified human PAPP-A /pro MBP heterotetrameric complex.
