Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response.
Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: In a sandwich FLISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 32pg/ml of recombinant murine IL-2 when used with biotinylated antigen affinity purified anti-murine IL-2 as the detection antibody at a concentration of at least 1-2ug/ml
Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2-4ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1-2ng/lane, under reducing conditions and 0.2-0.4ng/lane, under non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse IL-2.
Clone # USB：14G12
Isotype：IgG2a,k
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse IL-2, expressed in E. coli.
