Produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions.
It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 500pg/ml of recombinant human IFN-gamma when used with biotinylated antigen affinity purified anti-human IFN-gamma as the detection antibody at a concentration of approximately 2-4ug/ml
Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40ug/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1-3ng/lane, under non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from cell culture.
Form：Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Specificity：Recognizes human IFN-g.
Clone # USB：14G7
Isotype：IgG1,k
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IFN-g, expressed in E. coli.
