Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells.
It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation.
Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation.
Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis.
There are two isoforms, alpha and beta, of vertebrate Hsp90.
Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Reecognizes the epitope EEEVE within N-terminal part of ubiquitously expressed human Hsp90 alpha and Hsp90 beta intracellular proteins with calculated Mw of 84.7kD and 83.3kD, respectively, however, migrating as 90kD bands under reducing SDS-PAGE conditions. Species Sequence Homology: bovine and mouse
Clone # USB：MBH90AB
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo
Immunogen：Synthetic peptide corresponding to EEVHHGEEEVEC within the N-terminal of human Hsp90.
