HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 10ug/mlFlow Cytometry: 1-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:Recognizes native surface-expressed human HLA-E, but not denatured heavy chain of HLA-E.
Clone # USB:MEM-E/07
Isotype:IgG1
Calc Applications Abbrev:FC IHC IP
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to HLA-E refolded with beta2-microglobulin and peptide.