CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I.
CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
The expression can be upregulated by IFN-γ stimulation.
CD64 binds IgG immune complex.
It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: ≤ 1ug labels 10e6 cells in 10ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes the EC3 epitope of human CD64. Preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Species Crossreactivity: chimpanzee, baboon, cynomolgus, rhesus.
Clone # USB：10.1
Isotype：IgG1,k
Calc Applications Abbrev：FC IHC
Calc Crossreactivity：Hu
Immunogen：Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
